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KMID : 0604520180440010023
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Journal of the Society of Cosmetic Scientists of Korea
2018 Volume.44 No. 1 p.23 ~ p.29
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Tuning the Stiffness of Dermal Fibroblast-encapsulating Collagen Gel by Sequential Cross-linking
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Jung Mun-Hee
Shin Sung-Gyu
Lim Jun-Woo
Han Sa-Ra
Kim Hee-Jin
Jeong Jae-Hyun
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Abstract
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In this study, sequential cross-linked collagen gels were successfully prepared with collagen, which is biomaterial, and acrylamide (AAm), which is a synthetic monomer. The elastic moduli (E) of cross-linked collagen gels were increased from 1.5 to 3.0 kPa by varying of AAm concentrations. In addition, human dermal fibroblasts were encapsulated into the porous pores introduced into the gels, and cell growth and behavior were investigated. Increasing E of the gels led to decreases in cell growth rate, while the cellular glycosaminoglycan (GAG) production level was elevated. Overall, the growth and cellular activity of skin cells were influenced by the extracellular matrix properties of the collagen gels. In conclusion, these results will be highly useful for designing reconstructive skins and various tissue engineering researches.
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KEYWORD
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collagen gel, sequential cross-linking, hydrogel, elastic modulus
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